Concentration Assay:Article Title: Monitoring hPSC Genomic Stability in the Chromosome 20q Region by ddPCR
Article Snippet: .. Droplet generation Reagents • ddPCRTM Supermix for Probes (No dUTP) (Cat. no. 1863023, Bio-Rad Laboratories, Hercules, CA, USA) • PrimePCRTM ddPCRTM Copy Number Assay: BCL2L1, Human, FAM (Unique Assay ID: dHsaCP1000156, Bio-Rad Laboratories, Hercules, CA, USA) • PrimePCRTM ddPCRTM Copy Number Assay: PVRL2, Human, HEX (Unique Assay ID: dHsaCP2506625, Bio-Rad Laboratories, Hercules, CA, USA) • Restriction Enzyme HaeIII, 10,000 units/ml concentration (Cat. no. R0108S, New England Biolabs, Ipswich, MA) • Nuclease-free water Cell lysis reagents (optional) • UltraPureTM 0.5 M EDTA, pH 8.0 (Cat. No 15575020, Thermo Fisher Scientific, Waltham, MA, USA) • UltraPureTM 1 M Tris-HCI, pH 8.0 (Cat. No 15568025, Thermo Fisher Scientific, Waltham, MA, USA) • TritonTM X-100 solution, ∼10% in H2O (Cat. No. 93443, MilliporeSigma, Darmstadt, Germany) • Proteinase K Solution (20 mg/mL), RNA grade (Cat. No 25530049, Thermo Fisher Scientific, Waltham, MA, USA) • DPBS, no calcium, no magnesium (Cat. No 14190250, Thermo Fisher Scientific, Waltham, MA, USA) Instruments • QX200TM Droplet Generator (Cat. no. 1864002, Bio-Rad Laboratories, Hercules, CA, USA) ◦ Includes Droplet Generator Cartridge Holder (Cat. no. 1863051) Consumables • 8-strip PCR tubes • DG8TM Cartridges for QX200TM/QX100TM Droplet Generator (Cat. no. 1864008, Bio-Rad Laboratories, Hercules, CA, USA) • DG8TM Gaskets for QX200TM/QX100TM Droplet Generator (Cat. no. 1863009, Bio-Rad Laboratories, Hercules, CA, USA) • Droplet Generation Oil for Probes (Cat. no. 1863005, Bio-Rad Laboratories, Hercules, CA, USA) • ddPCRTM Buffer Control for Probes (Cat. no. 1863052, Bio-Rad Laboratories, Hercules, CA, USA) Optional Droplet Transfer, Plate Sealing and Thermocycling Instruments • PX1TM PCR plate sealer (Cat. no. 181-4000, Bio-Rad Laboratories, Hercules, CA, USA) • Thermocycler with 96–deep well reaction mod- ule Consumables • ddPCRTM 96-Well Plates (Cat. no. 12001925, Bio-Rad Laboratories, Hercules, CA, USA) • PCR Plate Heat Seal, foil, pierceable (Cat. no. 1814040, Bio-Rad Laboratories, Hercules, CA, USA) Droplet reading Instruments • QX200TM Droplet Reader (Cat. no. 1864003, Bio-Rad Laboratories, Hercules, CA, USA) This protocol has not been tested with the QX100 system, but we expect the procedure and results to be the same. .. ◦ Includes QuantaSoftTM Software (Version 1.7.4) Consumables • ddPCRTM Droplet Reader Oil (Cat. no. 1863004, Bio-Rad Laboratories, Hercules, CA, USA)
Lysis:Article Title: Monitoring hPSC Genomic Stability in the Chromosome 20q Region by ddPCR
Article Snippet: .. Droplet generation Reagents • ddPCRTM Supermix for Probes (No dUTP) (Cat. no. 1863023, Bio-Rad Laboratories, Hercules, CA, USA) • PrimePCRTM ddPCRTM Copy Number Assay: BCL2L1, Human, FAM (Unique Assay ID: dHsaCP1000156, Bio-Rad Laboratories, Hercules, CA, USA) • PrimePCRTM ddPCRTM Copy Number Assay: PVRL2, Human, HEX (Unique Assay ID: dHsaCP2506625, Bio-Rad Laboratories, Hercules, CA, USA) • Restriction Enzyme HaeIII, 10,000 units/ml concentration (Cat. no. R0108S, New England Biolabs, Ipswich, MA) • Nuclease-free water Cell lysis reagents (optional) • UltraPureTM 0.5 M EDTA, pH 8.0 (Cat. No 15575020, Thermo Fisher Scientific, Waltham, MA, USA) • UltraPureTM 1 M Tris-HCI, pH 8.0 (Cat. No 15568025, Thermo Fisher Scientific, Waltham, MA, USA) • TritonTM X-100 solution, ∼10% in H2O (Cat. No. 93443, MilliporeSigma, Darmstadt, Germany) • Proteinase K Solution (20 mg/mL), RNA grade (Cat. No 25530049, Thermo Fisher Scientific, Waltham, MA, USA) • DPBS, no calcium, no magnesium (Cat. No 14190250, Thermo Fisher Scientific, Waltham, MA, USA) Instruments • QX200TM Droplet Generator (Cat. no. 1864002, Bio-Rad Laboratories, Hercules, CA, USA) ◦ Includes Droplet Generator Cartridge Holder (Cat. no. 1863051) Consumables • 8-strip PCR tubes • DG8TM Cartridges for QX200TM/QX100TM Droplet Generator (Cat. no. 1864008, Bio-Rad Laboratories, Hercules, CA, USA) • DG8TM Gaskets for QX200TM/QX100TM Droplet Generator (Cat. no. 1863009, Bio-Rad Laboratories, Hercules, CA, USA) • Droplet Generation Oil for Probes (Cat. no. 1863005, Bio-Rad Laboratories, Hercules, CA, USA) • ddPCRTM Buffer Control for Probes (Cat. no. 1863052, Bio-Rad Laboratories, Hercules, CA, USA) Optional Droplet Transfer, Plate Sealing and Thermocycling Instruments • PX1TM PCR plate sealer (Cat. no. 181-4000, Bio-Rad Laboratories, Hercules, CA, USA) • Thermocycler with 96–deep well reaction mod- ule Consumables • ddPCRTM 96-Well Plates (Cat. no. 12001925, Bio-Rad Laboratories, Hercules, CA, USA) • PCR Plate Heat Seal, foil, pierceable (Cat. no. 1814040, Bio-Rad Laboratories, Hercules, CA, USA) Droplet reading Instruments • QX200TM Droplet Reader (Cat. no. 1864003, Bio-Rad Laboratories, Hercules, CA, USA) This protocol has not been tested with the QX100 system, but we expect the procedure and results to be the same. .. ◦ Includes QuantaSoftTM Software (Version 1.7.4) Consumables • ddPCRTM Droplet Reader Oil (Cat. no. 1863004, Bio-Rad Laboratories, Hercules, CA, USA)
Polymerase Chain Reaction:Article Title: Monitoring hPSC Genomic Stability in the Chromosome 20q Region by ddPCR
Article Snippet: .. Droplet generation Reagents • ddPCRTM Supermix for Probes (No dUTP) (Cat. no. 1863023, Bio-Rad Laboratories, Hercules, CA, USA) • PrimePCRTM ddPCRTM Copy Number Assay: BCL2L1, Human, FAM (Unique Assay ID: dHsaCP1000156, Bio-Rad Laboratories, Hercules, CA, USA) • PrimePCRTM ddPCRTM Copy Number Assay: PVRL2, Human, HEX (Unique Assay ID: dHsaCP2506625, Bio-Rad Laboratories, Hercules, CA, USA) • Restriction Enzyme HaeIII, 10,000 units/ml concentration (Cat. no. R0108S, New England Biolabs, Ipswich, MA) • Nuclease-free water Cell lysis reagents (optional) • UltraPureTM 0.5 M EDTA, pH 8.0 (Cat. No 15575020, Thermo Fisher Scientific, Waltham, MA, USA) • UltraPureTM 1 M Tris-HCI, pH 8.0 (Cat. No 15568025, Thermo Fisher Scientific, Waltham, MA, USA) • TritonTM X-100 solution, ∼10% in H2O (Cat. No. 93443, MilliporeSigma, Darmstadt, Germany) • Proteinase K Solution (20 mg/mL), RNA grade (Cat. No 25530049, Thermo Fisher Scientific, Waltham, MA, USA) • DPBS, no calcium, no magnesium (Cat. No 14190250, Thermo Fisher Scientific, Waltham, MA, USA) Instruments • QX200TM Droplet Generator (Cat. no. 1864002, Bio-Rad Laboratories, Hercules, CA, USA) ◦ Includes Droplet Generator Cartridge Holder (Cat. no. 1863051) Consumables • 8-strip PCR tubes • DG8TM Cartridges for QX200TM/QX100TM Droplet Generator (Cat. no. 1864008, Bio-Rad Laboratories, Hercules, CA, USA) • DG8TM Gaskets for QX200TM/QX100TM Droplet Generator (Cat. no. 1863009, Bio-Rad Laboratories, Hercules, CA, USA) • Droplet Generation Oil for Probes (Cat. no. 1863005, Bio-Rad Laboratories, Hercules, CA, USA) • ddPCRTM Buffer Control for Probes (Cat. no. 1863052, Bio-Rad Laboratories, Hercules, CA, USA) Optional Droplet Transfer, Plate Sealing and Thermocycling Instruments • PX1TM PCR plate sealer (Cat. no. 181-4000, Bio-Rad Laboratories, Hercules, CA, USA) • Thermocycler with 96–deep well reaction mod- ule Consumables • ddPCRTM 96-Well Plates (Cat. no. 12001925, Bio-Rad Laboratories, Hercules, CA, USA) • PCR Plate Heat Seal, foil, pierceable (Cat. no. 1814040, Bio-Rad Laboratories, Hercules, CA, USA) Droplet reading Instruments • QX200TM Droplet Reader (Cat. no. 1864003, Bio-Rad Laboratories, Hercules, CA, USA) This protocol has not been tested with the QX100 system, but we expect the procedure and results to be the same. .. ◦ Includes QuantaSoftTM Software (Version 1.7.4) Consumables • ddPCRTM Droplet Reader Oil (Cat. no. 1863004, Bio-Rad Laboratories, Hercules, CA, USA)
Control:Article Title: Monitoring hPSC Genomic Stability in the Chromosome 20q Region by ddPCR
Article Snippet: .. Droplet generation Reagents • ddPCRTM Supermix for Probes (No dUTP) (Cat. no. 1863023, Bio-Rad Laboratories, Hercules, CA, USA) • PrimePCRTM ddPCRTM Copy Number Assay: BCL2L1, Human, FAM (Unique Assay ID: dHsaCP1000156, Bio-Rad Laboratories, Hercules, CA, USA) • PrimePCRTM ddPCRTM Copy Number Assay: PVRL2, Human, HEX (Unique Assay ID: dHsaCP2506625, Bio-Rad Laboratories, Hercules, CA, USA) • Restriction Enzyme HaeIII, 10,000 units/ml concentration (Cat. no. R0108S, New England Biolabs, Ipswich, MA) • Nuclease-free water Cell lysis reagents (optional) • UltraPureTM 0.5 M EDTA, pH 8.0 (Cat. No 15575020, Thermo Fisher Scientific, Waltham, MA, USA) • UltraPureTM 1 M Tris-HCI, pH 8.0 (Cat. No 15568025, Thermo Fisher Scientific, Waltham, MA, USA) • TritonTM X-100 solution, ∼10% in H2O (Cat. No. 93443, MilliporeSigma, Darmstadt, Germany) • Proteinase K Solution (20 mg/mL), RNA grade (Cat. No 25530049, Thermo Fisher Scientific, Waltham, MA, USA) • DPBS, no calcium, no magnesium (Cat. No 14190250, Thermo Fisher Scientific, Waltham, MA, USA) Instruments • QX200TM Droplet Generator (Cat. no. 1864002, Bio-Rad Laboratories, Hercules, CA, USA) ◦ Includes Droplet Generator Cartridge Holder (Cat. no. 1863051) Consumables • 8-strip PCR tubes • DG8TM Cartridges for QX200TM/QX100TM Droplet Generator (Cat. no. 1864008, Bio-Rad Laboratories, Hercules, CA, USA) • DG8TM Gaskets for QX200TM/QX100TM Droplet Generator (Cat. no. 1863009, Bio-Rad Laboratories, Hercules, CA, USA) • Droplet Generation Oil for Probes (Cat. no. 1863005, Bio-Rad Laboratories, Hercules, CA, USA) • ddPCRTM Buffer Control for Probes (Cat. no. 1863052, Bio-Rad Laboratories, Hercules, CA, USA) Optional Droplet Transfer, Plate Sealing and Thermocycling Instruments • PX1TM PCR plate sealer (Cat. no. 181-4000, Bio-Rad Laboratories, Hercules, CA, USA) • Thermocycler with 96–deep well reaction mod- ule Consumables • ddPCRTM 96-Well Plates (Cat. no. 12001925, Bio-Rad Laboratories, Hercules, CA, USA) • PCR Plate Heat Seal, foil, pierceable (Cat. no. 1814040, Bio-Rad Laboratories, Hercules, CA, USA) Droplet reading Instruments • QX200TM Droplet Reader (Cat. no. 1864003, Bio-Rad Laboratories, Hercules, CA, USA) This protocol has not been tested with the QX100 system, but we expect the procedure and results to be the same. .. ◦ Includes QuantaSoftTM Software (Version 1.7.4) Consumables • ddPCRTM Droplet Reader Oil (Cat. no. 1863004, Bio-Rad Laboratories, Hercules, CA, USA)
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